[Histonet] Low temp Antigen Retrieval for Bone Sections

From:Kim Merriam

Hello All,
I will be performing some IHC on mouse femurs (about 12 or so different antibodies).  In the past, I have had a lot of trouble keeping these sections on the slides during HIER.  I did a quick search on the archives and there were lots of suggestions, but nothing definitive.
I was reading an article about "low temp AR", overnight at 60C in TEG buffer, pH 9.0. Has anyone tried this method?  Also - what does TEG buffer stand for?
Any help would be greatly appreciated.

Kim Merriam
Cambridge, MA
Do You Yahoo!?
Tired of spam?  Yahoo! Mail has the best spam protection around 
Histonet mailing list

<< Previous Message | Next Message >>