[Histonet] Low temp Antigen Retrieval for Bone Sections
I will be performing some IHC on mouse femurs (about 12 or so different antibodies). In the past, I have had a lot of trouble keeping these sections on the slides during HIER. I did a quick search on the archives and there were lots of suggestions, but nothing definitive.
I was reading an article about "low temp AR", overnight at 60C in TEG buffer, pH 9.0. Has anyone tried this method? Also - what does TEG buffer stand for?
Any help would be greatly appreciated.
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