[Histonet] Frozen tissue washing from slide
I'm hoping someone can give me some idea why frozen muscle sections would
suddenly start washing away from slide while trying to do PAS w/wo stain?
Other muscle panel stains do okay in staining process.....just the PAS w/wo is
recently having problems.
-We use superfrost plus slides
-Sections are fixed in 10% formalin prior to stain
-Most water rinses are DI. Only one tap water rinse step right after
-Does periodic acid have to be room temp prior to staining?
-Could Shiffs reagent be too cold?
-does temperature of water rinses affect adherance of sections to slides?
-We use a 10% acid water rinse after heme staining. I recall if acid water
is made incorrectly, tissue sections could wash much like they do if acid
water in H+E stain is too strong.
Thanks for any thoughts and help.
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