Arvind,

         Gelatine.  =  1g.
         Chrome Alum.  =  0.1g.  (Chromic potassium sulphate)
         Distilled water.  =  200ml.
         Dissolve the gelatine in the water using gentle heat then 
add the chrome alum. Coat the slides by dipping into the solution, 
draining off excess then drying in an oven. For large numbers of 
slides I keep clean staining racks specifically for subbing.

Ian.




>can any one pleas tell protocol for subbing slides with gelatin with 
>some extra attachment property actually my tissue are comming off 
>from the slides
>
>
>
>thanks
>
>
>
>Arvind Singh Pundir
>
>National Brain Rsearch Centre
>
>gurgaon, INDIA
>
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Dr. Ian Montgomery,
Histotechnology,
IBLS Support Services,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07623 975451
e-mail: ian.montgomery@bio.gla.ac.uk  


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