Re: [Histonet] Image Analysis
The intensity of DAB staining does not usually
indicate the quantity or concentration of an
antigen in an immunoperoxidase section. There
are even circumstances in which the amount of oxidized
DAB can vary inversely with the local concentration
of the antigen. In general: enzyme amplification
can detect and localize very small amounts of
some antigens, but the intensity of staining has
no quantitative significance.
There is a large body of peer-reviewed literature
in the field of quantitative immunohistochemistry.
Optical measurements correlate with antigen
concentration only with the simplest methods.
Anatomy Dept, UWO
Laurie Reilly wrote:
> >Hello Histonetters,
> Can anyone recommend an image analysis system which can measure the
> intensity of DAB staining in an
> immunohistochemical staining procedure?
> Thanks and regards, Laurie.
> Mr.Laurie Reilly Ph 07 4781 4468
> School of Veterinary & Biomedical Science Fax 07 4779 1526
> James Cook University
> Townsville Qld.
> 4811 firstname.lastname@example.org
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