RE: [Histonet] H&E

From:"Rogerson Kemlo (ELHT) Pathology"

You don't have an acid/ alcohol step? Either use a fresh progressive
haematoxylin step or introduce an acid/ alcohol step to remove the
'overstaining', then 'blue'.

-----Original Message-----
[] On Behalf Of Enniss,
Sent: 07 September 2005 14:35
To: ''
Subject: [Histonet] H&E

I have been working on getting a new staining protocol or new
My supervisor does not like the blue  background and she thinks we can
better than Harris. I am doing a demo on a linear stainer. I want to try
lowering our times down to 30 seconds and using Harris with acid.
6 stations-xylene
2 stations-100%
1 station-95%
2 stations-running tap water
2 stations-Hematoxylin
3 stations-tap water
1 station-80%
1 station-Eosin
2 Station-95%
3 Station-100%
2 Stations-Xylene
Does anyone else have any better ideas for an automated stainer or
stainer. If you could send protocols that would be useful.
Thanks so much.
Angel C. Enniss, HT (ASCP)
World Wide Safety Sciences AA
Tel 734 622 3154
Fax 734 622 3866

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