RE: [Histonet] C4d

From:"Drew Sally A."

We are using the Biogenesis C4d at 1:600 after 3 min HIER w/with pH 8.0
EDTA buffer in BioCare's DeCloaking Chamber.  On our Ventana we use 32
min antibody titration with an amplification step.

-----Original Message-----
[] On Behalf Of Poteete,
Jacquie A.
Sent: Friday, September 09, 2005 11:37 AM
To: ''
Subject: [Histonet] C4d

Does anyone have information they would be willing to share about C4d on
FFPE tissue?  I would especially be interested your methods of antigen
retrieval with times, dilution used, and antibody incubation time.  Any
other comments would be welcome.  Thanks in advance.

Jacquie Poteete MT(ASCP)QIHC
Lead Technologist, IHC Laboratory
Saint Francis Hospital, Tulsa OK.

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