[Histonet] (no subject)
I am trying to stain for motor neurons on frozen 8um sections for LCM. I
have been working with the .5% Cresyl Violet Acetate stain (Carson's version
of Vacca's stain) and all of it's derivatives. I am trying to counter the
differentiation of the alcohols used to completely dehydrate the sections
and leaving very little stain left. I am aiming for RNA preservation, so
length of time in the stain is important. I would appreciate any tips.
Thanks,
Patrick Laurie HT (ASCP)
Neurogenomics Laboratory
Benaroya Research Institute
1201 9th Ave
Seattle, Wa 98101
(206) 341-0681
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