[Histonet] B-Galactosidase Immuno Fluorescence
|From:||"Wragg, Andrew (NIH/NHLBI)" |
I am trying to stain mouse femoral artery OCT frozen sections with anti B
Galactosiadase antibodies for immuno fuorescent imaging.
I am using the ROSA26 mouse which expresses B Gal as my positive control.
I have not managed to find an antibody or set of conditions that work. I
ab6161, ABCAM, Rabbit polyclonal
MAB3468, Chemicon, Mouse monoclonal
I have tried paraformaldehyde 4%, ice cold methanol and methanol/acetone
I have no problem with X Gal staining the tissues.
Many thnaks for any help in advance
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