Re: [Histonet] endogenous peroxidase blocking protocol

From:Amy Porter

Anne - maybe try 0.03% in methanol.  It works pretty well for me with post fixed frozen sections of human tonsil.  

Ana Florentin  wrote:Hi,
Does anybody know a good protocol for blocking the
endogenous peroxidase? 
I am performing immunoperoxidase staining on rat
spleen frozen sections and I have tried to block
endogenous peroxidase with H2O2, 1% and 0.3% in PBS, 
half hour before the first antibody but is seemed to
be a too high concentration, as the tissue was
Thanks for help!

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Amy S.Porter, HT(ASCP) 
Michigan State University 
Department of Physiology 
Division of Human Pathology 
College of Human Medicine

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