Re: [Histonet] endogenous peroxidase blocking protocol
Anne - maybe try 0.03% in methanol. It works pretty well for me with post fixed frozen sections of human tonsil.
Ana Florentin wrote:Hi,
Does anybody know a good protocol for blocking the
endogenous peroxidase?
I am performing immunoperoxidase staining on rat
spleen frozen sections and I have tried to block
endogenous peroxidase with H2O2, 1% and 0.3% in PBS,
half hour before the first antibody but is seemed to
be a too high concentration, as the tissue was
destroyed.
Thanks for help!
anne
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Amy S.Porter, HT(ASCP)
Michigan State University
Department of Physiology
Division of Human Pathology
College of Human Medicine
portera203@yahoo.com
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