Dear Inga,
For double staining such markers, you can first stain the antibody that does not require heat. After the chromogen step, do your heat retrieval, and then stain with the next primary Ab.
Jo

>>> "Inga Hansson"  09/22/04 07:05AM >>>
Hi everyone!

If I want to do a double staining using one antibody that requires 
HIER and another that will stain everything if heated........what do 
I do?
Can anyone help me?

Thanks !

Inga
-- 


Inga Hansson
dept. neuroscience, div. neurobiology
PO Box 587
Biomedical Centre
Husargatan 3
S-751 23
Uppsala
SWEDEN

phone:+46-18-4714384
fax: +46-18-559017

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