Re: [Histonet] Processing lung tissue after pulmonary function tests
It seems to me that a solvent is a solvent is a solvent! The plastic
beads (polystyrene I suppose?) will dissolve in which ever solvent is
used to process the tissue.
Is frozen sectioning out of the question for some reason?
From: "Randolph-Habecker, Julie"
Date sent: Mon, 27 Sep 2004 09:11:52 -0700
Subject: [Histonet] Processing lung tissue after pulmonary function tests
> I am working with an investigator who is studying lung function after lung
> transplantation. We would like to look at FFPE lung tissue after the subject
> has had lung function tests which involve fluorescent plastic beads of
> varying size - the largest being 15 microns. I am concerned that the normal
> processing could melt the beads and cause artifact. Someone in another lab
> suggested that we use Histoclear II rather than Xylene.
> Does anyone have experience in processing tissue like this? Please give me
> some information on how to handle this tissue.
> Julie Randolph-Habecker, Ph.D.
> Experimental Histopathology Shared Resources
> Fred Hutchinson Cancer Research Center
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