Re: [Histonet] Processing lung tissue after pulmonary function tests

From:"Greg Dobbin"

Hi Julie,
It seems to me that a solvent is a solvent is a solvent! The plastic 
beads (polystyrene I suppose?) will dissolve in which ever solvent is 
used to process the tissue. 

Is frozen sectioning out of the question for some reason?

From:           	"Randolph-Habecker, Julie" 
To:             	"''" 
Date sent:      	Mon, 27 Sep 2004 09:11:52 -0700
Subject:        	[Histonet] Processing lung tissue after pulmonary function tests

> Histonetters,
> I am working with an investigator who is studying lung function after lung
> transplantation. We would like to look at FFPE lung tissue after the subject
> has had lung function tests which involve fluorescent plastic beads of
> varying size - the largest being 15 microns. I am concerned that the normal
> processing could melt the beads and cause artifact. Someone in another lab
> suggested that we use Histoclear II rather than Xylene.
> Does anyone have experience in processing tissue like this? Please give me
> some information on how to handle this tissue.
> Thanks!
> Julie
> Julie Randolph-Habecker, Ph.D.
> Experimental Histopathology Shared Resources
> Fred Hutchinson Cancer Research Center
> 1100 Fairview Ave. N, G1-300
> PO Box 19023
> Seattle, WA 98109-1024
> Tel: (206) 288-1187
> FAX: (206) 288-1345
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Greg Dobbin
Pathology Lab
Atlantic Veterinary College, U.P.E.I.
550 University Ave.
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