Re: [Histonet] Perfusion Fixation of Mouse Hearts

From:Phillip Huff

We currently perform routine mouse perfusions using paraformaldehyde as our fixative. We place a needle into the left ventricle of the heart and perfuse at 120 mmHg for 2-3 min, however we need to clamp the heart to the needle. I can forward you our detailed protocol for mouse perfusions, however you may have to adapt the procedure depending on the area of the heart you are interesting in analyzing. If a small hole at the apex of the heart is no problem then this protocol should work for you.

Christopher Jackson  wrote:
Hi everyone,

I am a research assistant at the Ottawa Heart Institute. I am working
with mice models.

I am supposed to learn how to perfusion fix a mouse heart and then embed
it into paraffin wax. Does anyone have a protocol that I could use, or
information regarding this technique?

Christopher Jackson
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