Re: [Histonet] Cresyl violet vs. cresyl violet acetate

From:"J. A. Kiernan"

> Isn't looking in the histonet almost the same as if looking it up in a book?

John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1

>  At least an effort is being made to look in the right place.
> What do you all think?
> I think the fact that Jennifer was able to find us means that she put some effort into it.  Shouldn't we reward her?
> I'll keep my answer on hold until we can find a consensus.  Good luck Jennifer.
> Juan C. Gutierrez, HT(ASCP)
> Histology Laboratory Supervisor
> (210)704-2533
> -----Original Message-----
> From: []
> Sent: Saturday, September 25, 2004 1:41 PM
> To: Jennifer Brielmaier;
> Subject: Re: [Histonet] Cresyl violet vs. cresyl violet acetate
> I have a question, concerning your "mystery" bottle -
> Do you mean that the teacher has no idea what it is (other than it might be
> a cresyl-family dye), and would like some idea/help on how to figure out
> what it is?
> Or, is this an assignment, where each student is supposed to find out the
> chemical nature of each of the dyes, and thus be able to chemically prove
> which dye it is. Where you are getting a grade for this.
> Be honest with us.
> If the teacher actually doesn't know, I think the Histonet community would
> be willing to help.
> If this is your homework assignment, I think that the Histonet community
> would be willing to refer you to a text book where you could look up the
> answers yourself, but that we would not be willing to do your homework for
> you.
> Sorry if I'm sounding a little edgy. I'm an instructor in histotechnology.
> All of my assignments are designed to teach students about histotechnology.
> But there is more to our field than just science. Some of my assignments are
> "team" assignments, where my students can pull together their knowledge and
> abilities to work on the assignment together. Part of what I'm assess is the
> ability of each student to contribute to a team. Some of my other
> assignments are "solo", where the person can use book, journals, internet,
> etc., but they cannot get the answers from their classmates or other techs.
> Part of what I'm assessing is the person's ability to find the answer on
> their own, not be told it. I need to know whether a person can problem-solve
> and troubleshoot on their own.
> Either way, there are text books and web pages out there with the
> information you need about your dyes. I'm sitting here with one of the books
> in my lab right now, which has the information.
> So - if your teacher needs the help, I am willing to help and quote from the
> pages.
> If you are supposed to find the answer, I'll let you know the name of the
> book. And you can look it up for yourself (and hopefully learn about this
> book, and learn about other dyes while leafing through this book).
> So, again, be honest, and let me know.
> Peggy A. Wenk, BA, BS, HTL(ASCP)SLS
> Program Director
> School of Histologic Technicians
> School of Histotechnologist
> William Beaumont Hospital
> Royal Oak, MI 48073
> ----- Original Message -----
> From: "Jennifer Brielmaier" 
> To: 
> Sent: Friday, September 24, 2004 11:43 AM
> Subject: [Histonet] Cresyl violet vs. cresyl violet acetate
> > Hello everyone,
> >
> > I am a first-year graduate student in a biopsychology program and am
> enrolled in a basic histology course this semester. Today in class we are
> going to learn how to make cresyl violet stain solutions. Our instructor has
> informed us that we have a "mystery" bottle in the lab; it is not known
> whether it is cresyl violet or cresyl violet acetate. Can anyone tell me
> whether there is a simple test that can be performed that will tell us which
> solution is in the bottle? Thanks very much.
> >
> > Jennifer
> >
> >
> > ---------------------------------
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