Re: [Histonet] Cresyl violet vs. cresyl violet acetate


I have a question, concerning your "mystery" bottle -

Do you mean that the teacher has no idea what it is (other than it might be
a cresyl-family dye), and would like some idea/help on how to figure out
what it is?

Or, is this an assignment, where each student is supposed to find out the
chemical nature of each of the dyes, and thus be able to chemically prove
which dye it is. Where you are getting a grade for this.

Be honest with us.

If the teacher actually doesn't know, I think the Histonet community would
be willing to help.

If this is your homework assignment, I think that the Histonet community
would be willing to refer you to a text book where you could look up the
answers yourself, but that we would not be willing to do your homework for

Sorry if I'm sounding a little edgy. I'm an instructor in histotechnology.
All of my assignments are designed to teach students about histotechnology.
But there is more to our field than just science. Some of my assignments are
"team" assignments, where my students can pull together their knowledge and
abilities to work on the assignment together. Part of what I'm assess is the
ability of each student to contribute to a team. Some of my other
assignments are "solo", where the person can use book, journals, internet,
etc., but they cannot get the answers from their classmates or other techs.
Part of what I'm assessing is the person's ability to find the answer on
their own, not be told it. I need to know whether a person can problem-solve
and troubleshoot on their own.

Either way, there are text books and web pages out there with the
information you need about your dyes. I'm sitting here with one of the books
in my lab right now, which has the information.

So - if your teacher needs the help, I am willing to help and quote from the

If you are supposed to find the answer, I'll let you know the name of the
book. And you can look it up for yourself (and hopefully learn about this
book, and learn about other dyes while leafing through this book).

So, again, be honest, and let me know.

Peggy A. Wenk, BA, BS, HTL(ASCP)SLS
Program Director
School of Histologic Technicians
School of Histotechnologist
William Beaumont Hospital
Royal Oak, MI 48073

----- Original Message -----
From: "Jennifer Brielmaier" 
Sent: Friday, September 24, 2004 11:43 AM
Subject: [Histonet] Cresyl violet vs. cresyl violet acetate

> Hello everyone,
> I am a first-year graduate student in a biopsychology program and am
enrolled in a basic histology course this semester. Today in class we are
going to learn how to make cresyl violet stain solutions. Our instructor has
informed us that we have a "mystery" bottle in the lab; it is not known
whether it is cresyl violet or cresyl violet acetate. Can anyone tell me
whether there is a simple test that can be performed that will tell us which
solution is in the bottle? Thanks very much.
> Jennifer
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