We have success using Sigma's Alkaline buffer solution, 1.5 M. Catalog #
A9226. If you want, I can attach the procedure to your email address.
Mary North, HT(ASCP)HTL
Neuromuscular Lab 
OHSU
Portland, OR

>>> "Vicki Gauch"  9/29/2004 8:54:37 AM >>>

Does anyone know what we can substitute for Barbitol in ATPase buffers
for muscle stains?  Any help you can give me would be greatly
appreciated..

Thanks,
Vicki Gauch
AMCH
Albany, NY

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