RE: [Histonet] vascular graft_sectioning difficulty

From:"Jack England"

Hmm.  If the tissue is coming out well, then processing and embedding seem 
fine; it sounds like the Dacron itself is brittle during cutting.  Are you 
observing the Dacron graft to be more brittle after processing than before?

I agree with Melanie about chilling the paraffin blocks or resin embedding.  
If you're not already doing so, maybe try cutting with a high profile blade, 
and/or a shallower blade angle?

--Aloha,
Jack England

>From: "Dr. Raghul" 
>To: joeamateur@hotmail.com,Histonet@lists.utsouthwestern.edu
>Subject: RE: [Histonet] vascular graft_sectioning difficulty
>Date: Thu, 16 Sep 2004 17:02:16 +0530
>
>Hello Aloha,
>
>    Our vascular graft material(DACRON) after fixing in 10% NBF, we
>dehydrate in ascending grades of alcohol(70,80,95,100%),cleared in 3
>changes of chloroform before embedding in paraffin wax (60*C MPT).
>
>    While cutting, the graft area  is powdering in the sections
>obtained.When it comes to the anastomising graft-host vessel part, while
>the vessel portion comes well, the graft as usual gets powdered .
>
>   This is our processing detail and the sectioning difficulty we
>face.Still if the details are insufficient please ask.
>
>regards
>raghul
>
>-----Original Message-----
>From: "Jack England" 
>To: raghul@sctimst.ker.nic.in, Histonet@lists.utsouthwestern.edu
>Date: Wed, 15 Sep 2004 08:54:40 -1000
>Subject: RE: [Histonet] vascular graft_sectioning difficulty
>
> > Can you provide a little more information about your processing, and a
> > little more detail about how the sections are crumbling (eg, tissue is
> > disaggregating, or the paraffin section is falling apart, etc)? We
> > regularly
> > section formalin-fixed, paraffin-embedded vascular grafts (ePTFE) at
> > 5-7m,
> > and it's working for us.  With a little more information, I might be
> > able to
> > help...
> >
> > Aloha,
> > Jack England
> > Tissue Genesis, Inc.
> > http://www.tissuegenesis.com
> >
> > >From: "Dr. Raghul" 
> > >To: histonet@lists.utsouthwestern.edu
> > >Subject: [Histonet] vascular graft_sectioning difficulty
> > >Date: Tue, 14 Sep 2004 16:56:23 +0530
> > >
> > >hello members,
> > >
> > >We cut 5 m thin sections of paraffin embedded vascular graft(Dacron)
> > in
> > >a rotary microtome with disposable blades but the senior tech tells
> > that
> > >the tissue sections are crumbling and forces us to go for thick
> > sections
> > >around 10m but again not with much success.
> > >
> > >  We can understand that there is not enough tissue adhereing to the
> > >graft and we are literally cutting the graft. But did anyone had such
> > >problems sectioning vascular grafts. Any suggestions?
> > >
> > >
> > >regards ,
> > >
> > >Raghul J
> > >scientist
> > >Histopathology- implant biology division
> > >Srichitra tirunal institute of medical sciences and technology
> > >Trivandrum -695 012
> > >Kerala, India
> > >
> > >
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> > >Histonet@lists.utsouthwestern.edu
> > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
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