RCC from novacastra is finicky. I went through the same process, trying
several HIER methods as well as enzymes.  Finally got it to work using a 1%
trypsin for 30 minutes at 37C.....can send you pictures if interested
(contact me off the list)

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
GUTIERREZ, JUAN
Sent: Wednesday, September 22, 2004 3:38 PM
To: Pat Zeitlow; histonet@pathology.swmed.edu
Subject: RE: [Histonet] rcc


I tried Novocastra's RCC-1 with no staining at all.  I even used several
different retrieval procedures.  I heard that DAKO's is pretty good.  Can
you share your search results with me.  Good luck.

Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
(210)704-2533


-----Original Message-----
From: Pat Zeitlow [mailto:pzeitlow@bbpllab.com]
Sent: Wednesday, September 22, 2004 1:44 PM
To: histonet@pathology.swmed.edu
Subject: [Histonet] rcc

I am looking to evaluate RCC (renal cell carcinoma) antibodies.  Is
anyone willing to share the clone, vendor and experiences for this
antibody.  I will be using it on paraffin embedded tissues.  TIA.

Pat

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