It's mouse tissue.  Here's the way I've been doing it recently. I've made changes from my original protocol.
 
Flex 70 (made from Flex 80).........1 hour (2 times)
Flex 95.......................................45 min
Flex 95.......................................1 hr
Flex 100.....................................1 hr (2 times)
ClearRite 3..................................1 hr @ Room Temp
ClearRite 3..................................1 hr @ 60 degrees
ClearRite 3/Parafin.......................1 hr @ 60 degrees
Parafin........................................Overnight (It's my stopping point)
Parafin........................................1 hr
Parafin........................................1 hr
 
Please let me know if I need to change anything.


Jennifer K. Sipes, RALAT
Sr. Laboratory Technician
Johns Hopkins University
Ross 929
720 Rutland Avenue
Baltimore, MD  21205
phone:  410-614-0131
cell:     443-413-0853
e-mail:  jengirl1014@yahoo.com
 









		
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