RE: [Histonet] double stainings
I agree with previous two posts. DAB for chromogen of non-HIER, then
whatever you want to contrast for second Ag. Technically one should use a
different enzyme system but, if the Ags are not co-localised, I might do
HRP/DAB/Cobalt/Nickel enhanced, giving brown and blue/black. The first
reporter molecule is sufficiently masked for it not to interfere. Obviously
do parallel sections with individual detection systems just for your/their
peace of mind. Only need to do that once….
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