RE: [Histonet] Water bath antigen retrieval

From:Tom McNemar

Wondering if this is your standard procedure for all antibodies?  I only
steam for 20 minutes with a 20 minute cool for all retrieval.  Place them in
buffer for 5 minutes then directly on the Autostainer.  They work

Just curious,

Tom Mc Nemar HT(ASCP)
Histology Supervisor
Licking Memorial Hospital
Newark, Ohio 43055

-----Original Message-----
From: Paula Pierce [] 
Sent: Thursday, September 23, 2004 2:05 PM
Subject: [Histonet] Water bath antigen retrieval

I use a Black and Decker rice steamer for antigen retrieval. About $30. Fill
the bottom with distilled water. Place the plastic basket in the steamer.
Place a container with your slides and citrate buffer in the basket so the
lid will fit. Turn the steamer on for 1 hour, then let cool for 25 minutes.
Rinse in distilled water slowly to finish cooling. Proceed with your IHC

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