RE: [Histonet] Mast Cell Stain other than Toluidine Blue

From:Tony Henwood

The alcian blue at pH 1 is a good demonstration method for mast cells.

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: Gayle Callis [] 
Sent: Friday, 1 October 2004 12:49 AM
To: Featherstone, Annette;
Subject: Re: [Histonet] Mast Cell Stain other than Toluidine Blue

No, but I have two methods that are very simple, both are T blue.  Churkian 
Schenk is my favorite, but have also used a method nicely passed on to me 
by Liz Chlipala out of Frieda Carson's book, a pH 4.3 T blue that takes 3 
to 5 minutes, rinse with water, air dry the section and mount.  This one is 
really simple and fast.   If you want, I can forward both to you privately.

At 05:37 AM 9/30/2004, you wrote:
>Does anyone have a simple Mast Cell Stain other than Toluidine Blue?
>Annette Featherstone HT/MLT
>-----Original Message-----
>From: hymclab []
>Sent: Wednesday, September 29, 2004 15:23
>To: 'Andrea Grantham';
>Subject: RE: [Histonet] Bouins substitute in Trichrome staining
>-----Original Message-----
>From: Andrea Grantham []
>Sent: Wednesday, September 29, 2004 10:38 AM
>Subject: [Histonet] Bouins substitute in Trichrome staining
>I attended a workshop at NSH last week on connective tissue staining and
>one of the attendees said they were using citrate buffer instead of bouins
>as a mordant. I couldn't catch up with her after the workshop to ask about
>the protocol. Is anybody out in histoland doing this? Would you mind
>sharing your protocol if you are?
>Andi Grantham
>: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
>: Sr. Research Specialist       University of Arizona               :
>: (office:  AHSC 4212)          P.O. Box 245044                     :
>: (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
>: (FAX:  520-626-2097)          (email:       :
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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