Dear Todd

We do a lot of experiments on eosinophilia in mouse, ensure to perfuse the lungs before processing, am sharing our protocol -

1.	We use 10% NBF to perfuse the mouse lungs and then subsequently proceed for immersion fixation for atleast a minimum of 24-48 hours before processing the mouse lung/s tissue.
2.	Next we do a routine H&E on sections cut at 5µ.

Hope this helps

Regards

Bharat Mani
Research Scientist (Biological Research)
Glenmark Research Centre
Plot No. A/607, TTC Ind. Area,
MIDC, Mahape
Navi Mumbai - 400 709
Ph.: +92-22-5590 2491/92 Ext.: 235
Fax: +91-22-5590 2318
Email: bharatm@glenmarkpharma.com  
Web: www.glenmarkpharma.com  


-----Original Message-----
From: Histology SLU [mailto:sluhisto@yahoo.com] 
Sent: Friday, September 17, 2004 1:12 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosinophils in mouse tissue


Does anyone out there have a protocol for staining eosinophils in mouse lung?  Thanks.
 
Todd

		
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