RE: [Histonet] Cresyl violet vs. cresyl violet acetate

From:"Barry R Rittman"

Histonet is a great resource and like most resources should be used
The discussions that we have recently seen reflect a general tendency in
our society for us to be fed complete pieces of information rather than
to provide a pathway for us to be able to follow a logical sequence of
events. I teach histology and it is obvious that most students expect to
be spoon fed information that has been examined, selected and summarized
by us for them. We are now in a situation where the impetus seems to be
to pass examinations rather than to learn material. (can you tell I have
spent 3 days marking examinations?)
I believe that part of this tendency is the rush to complete tasks and
with so many demands on our time everything seems to be prioritized. The
other problem is that with so many resources it is often difficult to
know the most appropriate source for information. I see many people
turning to Histonet because they have been thrown into a situation
without adequate training or complete instruction as to the final
outcome that is desired. I feel great sympathy for these individuals.
This is akin in some ways to asking individuals who have never painted
to produce a full length self portrait using acrylics. 
I strongly believe that Histonet shines in providing these individuals
with guidance as to where to look. I do not think that giving them a
complete final answer is as beneficial as providing them with general
directions and appropriate sites to look. It is always very tempting to
provide a final answer but I feel that this does not necessarily help
the learning process of such individuals.
To this end I suggest that in response to this type of questions that
general direction be given and that emphasis be placed on directly
contacting those who have submitted answers until the problem is solved.
Once solved to then supply the final answer to Histonet so that we can
all benefit.


-----Original Message-----
[] On Behalf Of Katri
Sent: Tuesday, September 28, 2004 10:03 AM
To: GUTIERREZ, JUAN; J. A. Kiernan;
Subject: Re: [Histonet] Cresyl violet vs. cresyl violet acetate

I love Histonet, but it is not better or worse than books and other
resources. It is different! It can create discussions, give you a right
direction for your problem solving and encourage further search for
information from books and other publications. It certainly has it's
in this fast moving technology of ours, but you have to have an open
when interpreting the responses.e
I enjoyed all five days of NSH Convention in Toronto and got reminded
again, that nothing is written in stone and advances in technology can
overthrow the old "proven" standards. Always keep an open mind...


Katri Tuomala
St.Joseph's Healthcare
Hamilton, Ontario, Canada

----- Original Message ----- 
To: "J. A. Kiernan" ;

Sent: Tuesday, September 28, 2004 6:30 AM
Subject: RE: [Histonet] Cresyl violet vs. cresyl violet acetate

You're right.  It's actually better.  With our busy schedules a lot of
we don't have the time to sit down and leaf through a bunch of books
for solutions to our problems.  It only takes a couple of minutes to
an e-mail and after completing some of our other tasks, it's nice to
back and find the answer waiting for you on the net.  My two cents.

Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor

-----Original Message-----
From: J. A. Kiernan []
Sent: Monday, September 27, 2004 3:40 PM
Subject: Re: [Histonet] Cresyl violet vs. cresyl violet acetate

> Isn't looking in the histonet almost the same as if looking it up in a

John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1

>  At least an effort is being made to look in the right place.
> What do you all think?
> I think the fact that Jennifer was able to find us means that she put
effort into it.  Shouldn't we reward her?
> I'll keep my answer on hold until we can find a consensus.  Good luck
> Juan C. Gutierrez, HT(ASCP)
> Histology Laboratory Supervisor
> (210)704-2533
> -----Original Message-----
> From: []
> Sent: Saturday, September 25, 2004 1:41 PM
> To: Jennifer Brielmaier;
> Subject: Re: [Histonet] Cresyl violet vs. cresyl violet acetate
> I have a question, concerning your "mystery" bottle -
> Do you mean that the teacher has no idea what it is (other than it
> a cresyl-family dye), and would like some idea/help on how to figure
> what it is?
> Or, is this an assignment, where each student is supposed to find out
> chemical nature of each of the dyes, and thus be able to chemically
> which dye it is. Where you are getting a grade for this.
> Be honest with us.
> If the teacher actually doesn't know, I think the Histonet community
> be willing to help.
> If this is your homework assignment, I think that the Histonet
> would be willing to refer you to a text book where you could look up
> answers yourself, but that we would not be willing to do your homework
> you.
> Sorry if I'm sounding a little edgy. I'm an instructor in
> All of my assignments are designed to teach students about
> But there is more to our field than just science. Some of my
> "team" assignments, where my students can pull together their
> abilities to work on the assignment together. Part of what I'm assess
> ability of each student to contribute to a team. Some of my other
> assignments are "solo", where the person can use book, journals,
> etc., but they cannot get the answers from their classmates or other
> Part of what I'm assessing is the person's ability to find the answer
> their own, not be told it. I need to know whether a person can
> and troubleshoot on their own.
> Either way, there are text books and web pages out there with the
> information you need about your dyes. I'm sitting here with one of the
> in my lab right now, which has the information.
> So - if your teacher needs the help, I am willing to help and quote
> pages.
> If you are supposed to find the answer, I'll let you know the name of
> book. And you can look it up for yourself (and hopefully learn about
> book, and learn about other dyes while leafing through this book).
> So, again, be honest, and let me know.
> Peggy A. Wenk, BA, BS, HTL(ASCP)SLS
> Program Director
> School of Histologic Technicians
> School of Histotechnologist
> William Beaumont Hospital
> Royal Oak, MI 48073
> ----- Original Message -----
> From: "Jennifer Brielmaier" 
> To: 
> Sent: Friday, September 24, 2004 11:43 AM
> Subject: [Histonet] Cresyl violet vs. cresyl violet acetate
> > Hello everyone,
> >
> > I am a first-year graduate student in a biopsychology program and am
> enrolled in a basic histology course this semester. Today in class we
> going to learn how to make cresyl violet stain solutions. Our
> informed us that we have a "mystery" bottle in the lab; it is not
> whether it is cresyl violet or cresyl violet acetate. Can anyone tell
> whether there is a simple test that can be performed that will tell us
> solution is in the bottle? Thanks very much.
> >
> > Jennifer
> >
> >
> > ---------------------------------
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> > - Register online to vote today!
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> > Histonet mailing list
> >
> >
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