[Histonet] re vimentin on mouse tissue
|From:||"PALMER Jason (SVHM)" |
We use Dako vimentin V9 as a human specific marker in human / mouse hybrid tissue. We have never ever seen it stain mouse tissue of any sort, and that's the way we like it! So, I'm not sure that you'll get it to stain (though I think I remember somebody on histonet in the past claiming that it does work with mouse....).
Bernard O'Brien Institute of Microsurgery
42 Fitzroy St, Fitzroy Victoria 3065
tel +61 3 9288 4018
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Date: Mon, 27 Sep 2004 15:55:06 -0700 (PDT)
From: Yolanda Maldonado
Subject: [Histonet] Vimentin on mouse tissue
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Does anyone out there has any experience with IHC stain for Vimentin on mouse tissue?
I have tried the V9 and 3B4 clone from different companies as well as the ARK kit and other polymers...Nothing seems to work for me!!
I have tried EDTA. CB, Reveal, LAB and enzymes as AR and I have also run human and other species along with my tissue to make sure my staining is working. Everything stains but the mice.
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