[Histonet] Red chromogens


I have been having some trouble with some Abs stained with New Fuchsin
and permanent red. Recently I was asked if I were using Levamisole in
the permanent red and if so how much? I was told too much Levamisole in
permanent red would result in no staining. I have used 1 drop per ml for
both New Fuchsin and permanent red, which is the appropriate amount.  We
have run both DABS and either permanent  red or New Fuchsin with the
same AB from the same vial with vastly different results. Any ideas?
Rena Fail  
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