[Histonet] Processing lung tissue after pulmonary function tests

From:"Randolph-Habecker, Julie"


I am working with an investigator who is studying lung function after lung
transplantation. We would like to look at FFPE lung tissue after the subject
has had lung function tests which involve fluorescent plastic beads of
varying size - the largest being 15 microns. I am concerned that the normal
processing could melt the beads and cause artifact. Someone in another lab
suggested that we use Histoclear II rather than Xylene.

Does anyone have experience in processing tissue like this? Please give me
some information on how to handle this tissue.



Julie Randolph-Habecker, Ph.D.
Experimental Histopathology Shared Resources
Fred Hutchinson Cancer Research Center
1100 Fairview Ave. N, G1-300
PO Box 19023
Seattle, WA 98109-1024
Tel: (206) 288-1187
FAX: (206) 288-1345

 This electronic message transmission contains information which may be
confidential or privileged. The information is intended to be for the
use of the individual or entity named above. If you are not the
intended recipient, be aware that any disclosure, copying, distribution
or use of the contents of this information is prohibited. If you have
received this electronic transmission in error, please leave a message
via telephone at (206) 288-6266, notify me by electronic reply, and
delete this message. Opinions and ideas in this message that do not
relate to official business are understood as neither given nor
endorsed by the Seattle Cancer Care Alliance. To view our complete
Notice of Privacy Practices, visit our web site at www.seattlecca.org.

Histonet mailing list

<< Previous Message | Next Message >>