Re: RE: [Histonet] Land snail dissection.
|From:||Jose Luis Palazon Fernandez |
If the snail is small I recomend you to fix the whole snail and after fixation, decalcify it with 10 % EDTA. then process and include the whole snail. Hope this help
El dia 08/09/2004 18:23 usted envio el siguiente mensaje:
>Date: 8 de Septiembre de 2004 18:23:01
>From: "Smith, Allen"
>Subject: RE: [Histonet] Land snail dissection.
>To: firstname.lastname@example.org, email@example.com
> Many centuries ago, I forced a snail out of its shell by shredding a pack of
> cigarettes into a pint of water and dropping the snail into it.
> Borradaile's THE INVERTEBRATA has instructions for dissecting the European
> garden snail Helix pomatia (pp. 604-610 in the 4th edition). The book is=0D
> out of print, but available used.
> Allen A. Smith, Ph.D.
> Professor of Anatomy
> Barry University
> School of Graduate Medical Sciences
> Podiatric Medicine and Surgery
> Miami Shores, Florida
> -----Original Message-----
> From: firstname.lastname@example.org
> [mailto:email@example.com] On Behalf Of Gayle Callis
> Sent: Wednesday, September 08, 2004 10:56 AM
> To: andromeda_tm; Histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Land snail dissection.
> Becaue of the hard calcified shell, dissection was still difficult. The =0D
> snail didn't always relax all the way out of the shell. I think we also =0D
> used another way to relax snail, but do not recall what was used (many =0D
> years ago!), as long as your method works, use it.
> Since we could never get a snail totally out of shell (they don't give it
> up easily!), we fixed snails whole, then decalcified them with shells
> intact, and processed them into paraffin. There is a "tooth" of some sort,
> I do not recall what my snail experts called it, but it can create problems
> during sectioning. It was very hard and caused section damage. There is
> always a possiblity that after you decalcify the shell, you can remove it
> very carefully to reach soft body parts. We had wonderful sections with =0D
> thin shell intact - a total histological preparation.
> We decalcified in 10 to 15% formic acid after the snail was totally fixed.
> At 04:48 AM 9/8/2004, you wrote:
> >Torino 08 September 2004
> >Hi all,
> >I am an amateur naturalist.
> >I like to study the histology of animal tissues by an optical microscope
> >in transmitted light.
> >I am interested to land (terrestrial) snails.
> >I know for relaxing the snail to use a solution of 50mM of MgCl2 by an =0D
> >injection (2 ml.) into the foot.
> >Could someone give me some detailed information how to proceed to the =0D
> >snail dissection?
> >Thank you.
> >With my Best Regards,
> Gayle Callis
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)
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