Re: [Histonet] specificity of Masson's trichrome

From:Geoff McAuliffe

Hi Carrie:

    While Masson's might (or might not) stain fibronectin and actin, no 
one (ie. reviewers of your work) will believe that it is specific 
without rigorous testing. If the results with Masson's do not agree with 
IHC, I cannot believe that anyone would throw out the IHC result in 
favor of the Masson's result.


Walker/Langdon wrote:

>Hi Histonetters,
>To clarify my question of the other day:  is it true that Masson's trichrome could potentially stain fibronectin and/or smooth muscle actin, especially if it is being overexpressed?  Is there a histochemical stain for fibronectin?  We have already successfully done IHC,but are trying to reconcile data obtained with a few different approaches.  We are mostly working in lung.
>Thanks to those who have already provided ideas!
>Carrie Langdon
>Dept. of Pathology
>McMaster University
>Hamilton, Ontario
>Histonet mailing list

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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