Re: [Histonet] p53 staining

From:Dana Settembre

Hello Laurie,
I use p53 on human tissue.  
The antibody is from DakoCytomation used at a 1 :150 dilution with a
colon cancer as a control.
I use DakoCytomations Target Retrieval Solution in a steamer for 40
Antibody is incubated at Room Temperature for 15 minutes then Dako's
LSAB2 kit for detection.
Hope this helps.
Dana Settembre
University Hospital - UMDNJ
Newark, NJ

>>> Laurie Reilly  9/6/2004 8:10:30 PM >>>
Dear All,
Does anyone have a protocol for doing p53 staining on Formalin Fixed 
Paraffin Embedded tissues.
We are trying various methods of antigen retrieval and incubation times
canine mammary tumour, and hope to be able to apply the technique to
mast cell tumours.

          Thanks for your consideration.   Regards, Laurie.

Mr.Laurie Reilly                                              Ph 07
4781 4468
Physiology & Pharmacology                           Fax  07 4779  1526
Aust.Inst.of Tropical Vet.& Animal Sc.
James Cook University
Townsville  Qld. 


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