Re: [Histonet] malarial pigment removal

From:"Barbara Bublava"

I use 5% ammonium hydroxide in tapwater for 20 minutes.
At first I used 70% Ethanol, it also did work but later I learned that
tapwater is also OK

It definetifely works on formalinpigment and according to literature it
should also remove malariapigment. We do several stains (HE, Goldner
(Tricrome), Elastica, Prussian blue, Van Gieson, Casons(SFOG), Ziehl nieson,
Gram, Kluver Barrera ...) and saw no changes, but we never tryed IHC or

Be shure to rinse well. remains of Ammonia can change the pH of
dyingsolutions (for example eosin)
 so they would get weaker and weaker

have a nice weekend
Barbara, Vienna

----- Original Message ----- 
From: "Bartlett, Jeanine" 
Sent: Thursday, September 09, 2004 8:04 PM
Subject: [Histonet] malarial pigment removal


What is the simplest and most frequently used procedure for removal of
malarial and/or formalin pigment?  I have heard concentrated sulfuric
acid works well but I do not know the time necessary in the acid.  I
have used saturated alcoholic picric acid in the past but no longer have
picric acid in my lab.  I have also heard of the Gridley technique.

I just wondered what everyone else is using.

Thanks in advance!

Jeanine Bartlett, HT(ASCP)
Centers for Disease Control and Prevention
Infectious Disease Pathology Activity
1600 Clifton Road, MS/G-32
Atlanta, GA 30333
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