Re: [Histonet] chromogen

From:Gayle Callis

DAKO has Permanent Red for alk phos IHC.  Chris van der Loos indicated DAKO 
product was very sensitive, and it also fluoresces.  Vector red is AP and 
also fluoresces. Have not tried Vector Red to compare with primary antibody 
concentrations.  These reds are more on dark pink red side, rather than 
orangish red of AEC, or Brick red of NOVA Red.

NOVA Red from Vector, but it is a brick red!!  Darker than AEC.

You can mount AEC from water, using Crystal mount, let this dry and then 
add a permanent mounting media.

At 11:39 AM 9/8/2004, you wrote:
>What is a good substitute for DAB using HRP, my investigator wants
>prettier red or green colors for a poster, something like he gets with
>FITC, but I don't want to do flourescence?  I know I could use AEC but I
>would prefer something more permanent.  Didn't Kirkgaurd and Perry come
>out with a permanent red chromogen for HRP?
>Histonet mailing list

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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