Re: [Histonet] Resin embedding-bone matrix

From:Gayle Callis

You did not say what plastic you are using.  Methyl methacrylate is 
considerably harder than glycol methacrylate.

At 04:00 PM 9/8/2004, you wrote:
>Hi all,
>I don't think this will work but.... I have been asked to resin embed some 
>bone that was exposed to treatments of high temps and pressures 
>etc.  Consequently I have been left only with the in-organic matrix.  I 
>cant decalcify it, or embed in paraffin because there will be nothing 
>left,  and I don't think it will cut even if I manage to get it into 
>resin.  One of the samples is just powder!!!  (Apparently they are looking 
>at a particular protein?)
>However the question is, should I make my resin mixture harder than normal 
>to accommodate the hard nature of the tissue?  Has anyone tried anything 
>similar???  Or do you have another medium I can embed into?
>Help and many thanks
>Caroline Stott
>Histology Service Unit
>University of Otago
>PO Box 913
>Dunedin, New Zealand
>Ph  (03) 479 7152
>Fax (03) 479 7136
>Histonet mailing list

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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