Fran,
Some antigenicity can be lost when cut slides are stored for a long time. Time varies for different antigens, and some are not effected at all. Some people think refrigeration, or even freezing controls slides lengthens storage time. I personally think that it's best to cut what you will use in a few months time. Trial and error.
Jo
>>> "Fran Lemons"  09/03/04 10:15AM >>>
Does anyone know why IHC control slides cannot be cut ahead of time?  I've done it at other facilities, but I have been told it is not permissible here, without any explanation.  Any ideas?
Also, has anyone ever heard of refrigerating pre-cut IHC control slides?  One person suggested to me that they stopped cutting them ahead of time because they were getting condensation on them & the stains wouldn't work.  Then she informed me that they were stored in the fridge.  Seems to me the condensation would have formed upon removing the slides from a cold environment to a room temp.
Your thoughts/theories are appreciated.
Fran Walker
Histology Technical Specialist
ETBH Knoxville


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