Re: [Histonet] Gomori chrome alum hematoxylin

From:Bryan Llewellyn

According to Gray it is:-

Water - 100 mL
Sulphuric acid, 5% aqu. - 2 mL
Potassium dichromate 5% aqu. - 2 mL
Chrome alum - 1.5 g
Hematoxylin - 0.5 g

Dissolve the alum and the hematoxylin each in 50 mL water.
Combine the two solutions.
Add the potassium dichromate and sulphuric acid.
Leave for 48 hours to ripen.

Bryan Llewellyn


Jose Luis Palazon Fernandez wrote:
> Dear list-members
> 
> 
> 
> Greetings. I can not find the recipe to prepare Gomori´s chrome alum hematoxylin. Could any of you help me please?
> 
> 
> 
> Thanks in advance
> 
> 
> 
> 
> 
> José Luis
> 
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