Re: [Histonet] GFP in a fixed brain tissue

From:Gayle Callis

Maybe, it is probably best to cut frozen sections, fix with 2% 
paraformaldehyde for 1 minute, rinse, and mount with Molecular Probes, 
Probe On antifade mounting media, ready to use.

Go to Clontech website and read Living Colours manual on some of these 

Their tech services is excellent.  This question has been discussed, even 
recently, on Histonet, you can search the archives at

At 07:12 PM 9/10/2004, you wrote:
>    Regards from a new entrants to histonet. I have GFP labelled
>parasites sitting in brain of my rats. I need to slice the brain and
>locate these parasites. Will GFP still be present if I use fixatives
>like formaldehyde or organic solvents like xylene? What fixing
>protocols are best for preserving GFP signal?
>Thanks in advance,
>Histonet mailing list

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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