Re: [Histonet] CD4 and CD8 in paraffin?

From:Gayle Callis

Teri et al,

You did not mention the species.  If the staining was on human duodenal 
biopsies (tough thing to do on a mouse!) rather than mouse tissue then 
these markers work. The Mouse remains a total bust!  Boo Hoo! Must be the 
critter aka species differences rather than living Down Under, eh!??

  At 08:49 AM 9/1/2004, you wrote:
>In this latest issue of Applied Immunohistochemistry and Molecular
>Morphology (AIMM 2004; 12:198-204) is an article in which CD4 and CD8
>are used in paraffin sections with antigen unmasking.  CD4 (clone 1F6,
>1:20 from Novocastra, Malvern, Australia) and CD8 (clone C8/144B, 1:200;
>Dako, Sydney, Australia) were done on routinely processed duodenal
>biopsies.  Maybe we're in the wrong hemisphere and that explains why it
>doesn't work for us?
>Teri Johnson
>Managing Director Histology Facility
>Stowers Institute for Medical Research
>1000 E. 50th St.
>Kansas City, Missouri  64110
>Histonet mailing list

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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