We antigen retrieve our synaptophysin in Citrate Buffer  ph6.0 in a steamer.
We set the steamer with the citrate in it for 70 minutes, at 20 minutes left
we add the slides. After it steams for the final 20 minutes we let it cool
for 20 minutes. We add amplifier and blocker too. Is your primary dilution
correct?
Annette Featherstone HT/MLT Kaleida Health.

-----Original Message-----
From: Joe Nocito [mailto:jnocito@satx.rr.com]
Sent: Wednesday, September 08, 2004 22:02
To: JAnorthernexp@cs.com; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] synaptophysin


Annette,
did you try increasing the time in cell conditioner? Have you tried to
amplify it?

Joe Nocito BS, HT(ASCP)QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX
----- Original Message -----
From: 
To: 
Sent: Wednesday, September 08, 2004 7:06 PM
Subject: [Histonet] synaptophysin


> anyone using a ventana stainer and antibodies have a retrieval method
using a
> decloker?  Ours stains very light.  Antibody is on for 32 mins we did have
it
> set at 16mins.  But increasing the time did not prove to make much
> difference.  Any other ideas??  The tissue is formalin fixed and paraffin
embedded.
> Thanks
>
> Annette
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> Histonet@lists.utsouthwestern.edu
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>



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