RE: [Histonet] Superfrost Slides

From:"Joe Nocito"

Bill,
we've had problems also. It could be that the slides might be too changed,
which is causing the reagents to bead up. Currently, we are using positive
charged slides from Statlab Medical Products, which I think they get from
Erie Scientific. Statlab's number is 1-800-442-3573. The may be able to
direct you to some one closer to you.

Joe Nocito, BS, HT(ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bill
Sinai
Sent: Monday, September 13, 2004 10:43 PM
To: histonet (E-mail)
Subject: [Histonet] Superfrost Slides



Is anyone having troubles with NO staining on some slides on the Ventana
Benchmark.
The biggest problem seems to be with the EDTA buffered CC1 retrieval
solution.
Not all slides are effected, just an occasional one, but we usually end up
with little or no staining with anything including the Haematoxylin.
We use Menzel Superforst Plus and Superfrost Plus Ultra, and both slides
have shown the effect.

Thanks
Bill Sinai
Laboratory Manager
Tissue Pathology, ICPMR
Westmead NSW 2145
Australia
Ph 02 9845 7774


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