RE: [Histonet] Question regarding 10% Neutral buffered formalin/Immuno

From:"Dunn-Jena, Patsy A"

Additional information.

The tissue is the palate/maxillary teeth of mice.  It is fixed in 10% NBF for 24 hours prior to starting to decal in a 0.25 M EDTA which takes about 4 weeks.  


-----Original Message-----
[]On Behalf Of
Dunn-Jena, Patsy A
Sent: Friday, September 10, 2004 9:49 AM
To: Histonet
Subject: [Histonet] Question regarding 10% Neutral buffered

I have a graduate student who has come to me with a question I cannot answer as I have limited Immunohistochemical experience.  One of her advisers wants her to go to using an EDTA decalcification recipe which contains the 10% NBF in it which is a recipe we used on all of his previous work.  She asked me about the NBF crosslinking her immuno sites as she has been using an EDTA decalcification only recipe.  Some input here would be nice so I can better explain it to her.

Patsy A. Dunn-Jena, RVT, LAT, HT (ASCP)
Mineralized Tissue and Histology Research Laboratory
Indiana University School of Dentistry
1121 W. Michigan Street, Room 238
Indianapolis, IN 46202

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