RE: [Histonet] Pre-cut controls

From:"Bauer, Karen"

We cut all of our controls ahead of time and have had no problems.  Controls
are tested before we use them and then of course are monitored when we
actually use them.  As far as I know, the Pathologists are happy with what
we have and I have not had to change our way of handling controls.  

After we cut the controls, we put them in slide boxes and leave them open to
air dry for a few hours or even overnight if cut late in the afternoon.  We
then place them in the cupboard and then use them when we need them.

Good Luck to you...

Karen Bauer HT(ASCP)
Histology Supervisor
Department of Pathology
Luther Hospital
Eau Claire, WI

-----Original Message-----
From: Joanne Mauger []
Sent: Friday, September 03, 2004 9:40 AM
Subject: Re: [Histonet] Pre-cut controls

Some antigenicity can be lost when cut slides are stored for a long time.
Time varies for different antigens, and some are not effected at all. Some
people think refrigeration, or even freezing controls slides lengthens
storage time. I personally think that it's best to cut what you will use in
a few months time. Trial and error.
>>> "Fran Lemons"  09/03/04 10:15AM >>>
Does anyone know why IHC control slides cannot be cut ahead of time?  I've
done it at other facilities, but I have been told it is not permissible
here, without any explanation.  Any ideas?
Also, has anyone ever heard of refrigerating pre-cut IHC control slides?
One person suggested to me that they stopped cutting them ahead of time
because they were getting condensation on them & the stains wouldn't work.
Then she informed me that they were stored in the fridge.  Seems to me the
condensation would have formed upon removing the slides from a cold
environment to a room temp.
Your thoughts/theories are appreciated.
Fran Walker
Histology Technical Specialist
ETBH Knoxville

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