RE: [Histonet] CD4 and CD8 in paraffin?


We run them all the time on the Benchmarks.  Ventana sells the primaries, probably from NCL, and we've gotten excellent results on GI bx.

Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor

My views are my own and do not reflect those of my employer.
Long live free speech!  "We have nothing to fear but fear itself".

-----Original Message-----
From: Johnson, Teri []
Sent: Wednesday, September 01, 2004 9:50 AM
To: Histonet
Subject: [Histonet] CD4 and CD8 in paraffin?

In this latest issue of Applied Immunohistochemistry and Molecular
Morphology (AIMM 2004; 12:198-204) is an article in which CD4 and CD8
are used in paraffin sections with antigen unmasking.  CD4 (clone 1F6,
1:20 from Novocastra, Malvern, Australia) and CD8 (clone C8/144B, 1:200;
Dako, Sydney, Australia) were done on routinely processed duodenal
biopsies.  Maybe we're in the wrong hemisphere and that explains why it
doesn't work for us?

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110

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