[Histonet] re freezing mouse embryos
IMHO, the morphology/histology of sections of unfixed, frozen embryos (
even after fixing the sections after cutting)is inferior to that of fixed,
then frozen, tisssues, but should still be easily recognisable. Either the
tissue is necrotic or that the freezing process is inadequate, leading to
ice-crystal artefact. I do fixed and unfixed embryos by the processes you
describe. What age are your embryos? Do you post-fix the sections? What in?
Best wishes
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