[Histonet] RE: Histonet Digest, Vol 10, Issue 22

From:DEllenburg2@stfrancishealth.org

Not sure if this is what you are looking far.  
 
CHROMIUM HEMATOXYLIN SOLUTION.
  
Hematoxylin, 1% aqueous solution................................   50.0 cc.
Chromium alum, 3% aqueous solution..............................   50.0 cc.
  
To 100 cc. of chromium hematoxylin add 0.1 gm. of potassium iodate.  
Boil until a deep blue.  The mixture is ripe immediately anc can be used
as long as a film with a metallic luster forms on its surface in a Coplin
jar.  Filter before use.  Hope this helps.  If you would like I will fax
this procedure to you. 
  
Deborah Ellenburg, HT (ASCP)
Histology Supervisor
Bon Secours St. Francis Health System
(phone)  864-255-1278
(fax)    864-2551664

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Today's Topics:

   1. Re: Block retention (LuAnn Anderson)
   2. Cutting controls (Cox, Jill)
   3. RE: Sulfur Granule Stain (Bill Blank)
   4. Gomori chrome alum hematoxylin (Jose Luis Palazon Fernandez)
   5. CD179 (Mildred Fail)


----------------------------------------------------------------------

Message: 1
Date: Wed, 15 Sep 2004 10:56:48 -0500
From: LuAnn Anderson 
Subject: Re: [Histonet] Block retention
To: "Pizi, Dave" ,
	
Message-ID: <5.2.0.9.0.20040915105049.00a3eec0@ander093.email.umn.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed

We have all slides and blocks dating back at least 30 years or more. We 
have received many requests over the years for slides that are older than 
10 years--so this is a very valid concern, IMHO. We have a separate store 
room where these slides and blocks are kept. Our Neuropathologist does not 
want anything discarded--don't know what we'll do when the storeroom fills 
up--but we will be able to pull slides/blocks if needed. I do agree that 
this policy should be reviewed.

LuAnn




At 11:31 AM 9/15/04 -0400, Pizi, Dave wrote:
>I hope this doesn't open a can of worms, but our medical director would 
>like to know how other labs are handling block and slide disposal when 
>there is a cancer center involved.  His concern is that even though we are 
>following CAP and state guidelines when we discard after 10 years, the 
>cancer center folks will request something older and we won't be able to 
>help them.  I can't think of a way to identify, retrieve and hold this 
>material that doesn't give me a headache.
>
>Thanks
>Dave Pizi
>Carolinas HealthCare System
>Charlotte, NC
>
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------------------------------

Message: 2
Date: Wed, 15 Sep 2004 09:09:04 -0700
From: "Cox, Jill" 
Subject: [Histonet] Cutting controls
To: 
Message-ID:
	
<05424658AAA6D441BBAFD9D9D98780B7044F0CB1@lcmmsg02.ca.providence.org>
Content-Type: text/plain;	charset="Windows-1252"


Hello Netters,
 I had previously posted a message on starting a control cutting business.
Well I did it!!!  Thank you for all your help. My website is the only thing
I have left to finish. If anyone needs any assistance in control slides or
just cutting blocks I would love to help you. Now you at least have one more
resource.    I hope this is ok content to post.        Thanks again... 



Jill Cox HT ascp
Pathology Supervisor
Little Company of Mary
4101 Torrance Blvd.
Torrance Ca. 90503
(310) 543-6736





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------------------------------

Message: 3
Date: Wed, 15 Sep 2004 11:27:02 -0500
From: Bill Blank 
Subject: RE: [Histonet] Sulfur Granule Stain
To: "Marshall Terry Dr,	Consultant Histopathologist"
	, 	"Paula Lucas"
,
	"Histonet (E-mail)" 
Message-ID: 
Content-Type: text/plain; charset="us-ascii" ; format="flowed"

At 4:02 PM +0100 9/15/04, Marshall Terry Dr,
	Consultant Histopathologist wrote:
>A tea strainer would likely have been better than ThinPrep:-)

LOL! I do use tea strainers in the gross room (American tea 
strainers...). Almost anything will stain them - an H&E is fine. They 
are PAS+, AFB+ Gram+ etc

It might be better to culture for actinomyces... If this is a serious 
possibility

As I recall...

BB
-- 
_____________________________
Bill Blank
http://kernunnos.com (Celtic studies and numismatics)
OBOD's Message board: http://www.druidry.org/board






------------------------------

Message: 4
Date: Wed, 15 Sep 2004 18:31:57 +0200 (CEST)
From: Jose Luis Palazon Fernandez 
Subject: [Histonet] Gomori chrome alum hematoxylin
To: histonet@lists.utsouthwestern.edu
Message-ID: <20040915163157.544262C3A2A@perceval.uca.es>
Content-Type: text/plain; charset="iso-8859-1"

Dear list-members

Greetings. I can not find the recipe to prepare Gomori┤s chrome alum
hematoxylin. Could any of you help me please?

Thanks in advance


JosÚ Luis



------------------------------

Message: 5
Date: Wed, 15 Sep 2004 12:56:25 -0400
From: "Mildred Fail" 
Subject: [Histonet] CD179
To: 
Message-ID: 
Content-Type: text/plain; charset=US-ASCII

Has anyone used this marker? IF so at what dilution
Thank
Rena Fail




------------------------------

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