[Histonet] RE: Histonet Digest, Vol 10, Issue 22
Not sure if this is what you are looking far.
CHROMIUM HEMATOXYLIN SOLUTION.
Hematoxylin, 1% aqueous solution................................ 50.0 cc.
Chromium alum, 3% aqueous solution.............................. 50.0 cc.
To 100 cc. of chromium hematoxylin add 0.1 gm. of potassium iodate.
Boil until a deep blue. The mixture is ripe immediately anc can be used
as long as a film with a metallic luster forms on its surface in a Coplin
jar. Filter before use. Hope this helps. If you would like I will fax
this procedure to you.
Deborah Ellenburg, HT (ASCP)
Bon Secours St. Francis Health System
Subject: Histonet Digest, Vol 10, Issue 22
Send Histonet mailing list submissions to
To subscribe or unsubscribe via the World Wide Web, visit
or, via email, send a message with subject or body 'help' to
You can reach the person managing the list at
When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
1. Re: Block retention (LuAnn Anderson)
2. Cutting controls (Cox, Jill)
3. RE: Sulfur Granule Stain (Bill Blank)
4. Gomori chrome alum hematoxylin (Jose Luis Palazon Fernandez)
5. CD179 (Mildred Fail)
Date: Wed, 15 Sep 2004 10:56:48 -0500
From: LuAnn Anderson
Subject: Re: [Histonet] Block retention
To: "Pizi, Dave" ,
Content-Type: text/plain; charset="us-ascii"; format=flowed
We have all slides and blocks dating back at least 30 years or more. We
have received many requests over the years for slides that are older than
10 years--so this is a very valid concern, IMHO. We have a separate store
room where these slides and blocks are kept. Our Neuropathologist does not
want anything discarded--don't know what we'll do when the storeroom fills
up--but we will be able to pull slides/blocks if needed. I do agree that
this policy should be reviewed.
At 11:31 AM 9/15/04 -0400, Pizi, Dave wrote:
>I hope this doesn't open a can of worms, but our medical director would
>like to know how other labs are handling block and slide disposal when
>there is a cancer center involved. His concern is that even though we are
>following CAP and state guidelines when we discard after 10 years, the
>cancer center folks will request something older and we won't be able to
>help them. I can't think of a way to identify, retrieve and hold this
>material that doesn't give me a headache.
>Carolinas HealthCare System
>This electronic message may contain information that is confidential
>and/or legally privileged. It is intended only for the use of the
>individual(s) and entity named as recipients in the message. If you are
>not an intended recipient of this message, please notify the sender
>immediately and delete the material from any computer. Do not deliver,
>distribute or copy this message, and do not disclose its contents or take
>any action in reliance on the information it contains. Thank you.
>Histonet mailing list
Date: Wed, 15 Sep 2004 09:09:04 -0700
From: "Cox, Jill"
Subject: [Histonet] Cutting controls
Content-Type: text/plain; charset="Windows-1252"
I had previously posted a message on starting a control cutting business.
Well I did it!!! Thank you for all your help. My website is the only thing
I have left to finish. If anyone needs any assistance in control slides or
just cutting blocks I would love to help you. Now you at least have one more
resource. I hope this is ok content to post. Thanks again...
Jill Cox HT ascp
Little Company of Mary
4101 Torrance Blvd.
Torrance Ca. 90503
This message is intended for the sole use of the addressee, and may contain
information that is privileged, confidential and exempt from disclosure
under applicable law. If you are not the addressee you are hereby notified
that you may not use, copy, disclose, or distribute to anyone the message or
any information contained in the message. If you have received this message
in error, please immediately advise the sender by reply email and delete
Date: Wed, 15 Sep 2004 11:27:02 -0500
From: Bill Blank
Subject: RE: [Histonet] Sulfur Granule Stain
To: "Marshall Terry Dr, Consultant Histopathologist"
, "Paula Lucas"
Content-Type: text/plain; charset="us-ascii" ; format="flowed"
At 4:02 PM +0100 9/15/04, Marshall Terry Dr,
Consultant Histopathologist wrote:
>A tea strainer would likely have been better than ThinPrep:-)
LOL! I do use tea strainers in the gross room (American tea
strainers...). Almost anything will stain them - an H&E is fine. They
are PAS+, AFB+ Gram+ etc
It might be better to culture for actinomyces... If this is a serious
As I recall...
http://kernunnos.com (Celtic studies and numismatics)
OBOD's Message board: http://www.druidry.org/board
Date: Wed, 15 Sep 2004 18:31:57 +0200 (CEST)
From: Jose Luis Palazon Fernandez
Subject: [Histonet] Gomori chrome alum hematoxylin
Content-Type: text/plain; charset="iso-8859-1"
Greetings. I can not find the recipe to prepare Gomori┤s chrome alum
hematoxylin. Could any of you help me please?
Thanks in advance
Date: Wed, 15 Sep 2004 12:56:25 -0400
From: "Mildred Fail"
Subject: [Histonet] CD179
Content-Type: text/plain; charset=US-ASCII
Has anyone used this marker? IF so at what dilution
Histonet mailing list
End of Histonet Digest, Vol 10, Issue 22
The information in this communication is intended to be confidential to the
Individual(s) and/or Entity to whom it is addressed. It may contain
information of a Privileged and/or Confidential nature, which is subject to
Federal and/or State privacy regulations. In the event that you are not the
intended recipient or the agent of the intended recipient, do not copy or
use the information contained within this communication, or allow it to be
read, copied or utilized in any manner, by any other person(s). Should this
communication be received in error, please notify the sender immediately
either by response e-mail or by phone at (864) 255-1000, and permanently
delete the original e-mail, attachment(s), and any copies.
Histonet mailing list
<< Previous Message | Next Message >>