[Histonet] IHC on Frozen Sections
Hello,
I am trying to do the T & B cells on frozen section tonsil for my IHC
exam
due at the end of this month. I have tried multiple procedures, and
have
not yet found one that makes me happy, or looks like the one in the
picture
at the IHC home page.
If someone could e-mail me their procedures (mostly from cutting to
antibody
application) it would be greatly appreciated.
By the way, my cryostat will not cut thinner than 4-6 microns.
Thanks in advance,
Rhonda Allen
VA Medical Center
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