Hi There

I know this topic has been around before, so forgive me!!

I am attempting doulble and then tripple fluorescent labelling. Do I 
need to add a fixation step between Primary antibodies, to secure the 
first Ag/Ab complex???

Any help with this proceedure will be greatly appreciated.

Many Thanks
Melanie.
-- 
Cardiovascular Research Unit
Div. of Cardiothoracic Surgery
Chris Barnard Building
University of Cape Town
Anzio Road
Observatory
7925
Republic of South Africa

Tel +27 21 406-6589
Cel +27 82 469-3352
Fax +27 21 448-5935

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