[Histonet] Annette, synaptophysin
I use a steamer (Black & Decker) for retrieval. I put the slides
into Cell Marque's Trilogy and steam for 20 min. Take the slides out of
the steamer and let cool for 10 min. Place slides into APK wash
(ventana) for 5 min. I incubate for 32 min. No amplification or
blocking. I have no problem with light staining.
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