How do you mount the tissue on the slide?
Donna
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Donna Kumiski HT (ASCP)
Medical College of Georgia
Dept. of Cellular Biology and Anatomy
CB 1202
Augusta, Georgia 30912-2000
(706) 721-6278
dkumiski@mail.mcg.edu
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Geoff McAuliffe <mcauliff@umdnj.edu> 9/18/2003 10:55:23 AM >>>
Hi Felix:
For mesentaries some people like a "spread" rather than a section.
Take out several loops of intestine and pin them out on a sheet of cork
or dental wax with a tiny bit of tension on the mesentary so it is flat.
Submerge in fix. You can process the whole "block" this way throught
alcohols and clearing, then cut out and mount the sheet of mesentary on
a slide without embedding. Yes it will be thicker than a section but you
will see a nice 3D tissue with capillaries etc.
Geoff
Felix.Rintelen@serono.com wrote:
Dear all,
Is there anybody who may give me advice how to make good sections of
parafin embedded mesentery (mouse)? How to prepare the tissue for embedding
and how to handle it to embed/orient it properly in the parafin block? My
concern is especially the soft consistency of the tissue making me feel
that the proper orientation in the block might be difficult.
Thank you very much in advance,
Felix
Felix Rintelen
Serono Pharmaceutical Research Institute
Geneva, Switzerland
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