Re: [Histonet] how to embed/section mesentery?

From:Geoff McAuliffe

    Assuming that the tissue is flat enough (from tension applied during fixation and processing) you can put cleared tissue on a slide and coverslip it. Of course, it was stained (which I forgot to mention, blush!) after fixation and before dehydration and clearing. We use such spreads to teach the histology of loose connective tissue to first year medical students. Probably not something a clinical lab would do.


Donna Kumiski wrote:
How do you mount the tissue on the slide?

Donna Kumiski     HT (ASCP)
Medical College of Georgia
Dept. of Cellular Biology and Anatomy
CB 1202
Augusta, Georgia   30912-2000   
(706) 721-6278

Geoff McAuliffe <> 9/18/2003 10:55:23 AM >>>
Hi Felix:

    For mesentaries some people like a "spread" rather than a section. 
Take out several loops of intestine and pin them out on a sheet of cork 
or dental wax with a tiny bit of tension on the mesentary so it is flat. 
Submerge in fix. You can process the whole "block" this way throught 
alcohols and clearing, then cut out and mount the sheet of mesentary on 
a slide without embedding. Yes it will be thicker than a section but you 
will see a nice 3D tissue with capillaries etc.

Geoff wrote:

Dear all,

Is there anybody who may give me advice how to make good sections of
parafin embedded mesentery (mouse)? How to prepare the tissue for embedding
and how to handle it to embed/orient it properly in the parafin block? My
concern is especially the soft consistency of the tissue making me feel
that the proper orientation in the block might be difficult.

Thank you very much in advance,


Felix Rintelen
Serono Pharmaceutical Research Institute
Geneva, Switzerland

S - This message contains confidential information and is intended only for the individual
named. If you are not the named addressee, you should not disseminate, distribute or copy
this e-mail. Please notify the sender immediately by e-mail if you have received this
e-mail by mistake and delete this e-mail from your system.
e-mail transmission cannot be guaranteed to be secure or error-free as information could be
intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain malware. The
presence of this disclaimer is not a proof that it was originated at Serono International S.A.
or one of its affiliates. Serono International S.A and its affiliates therefore do not accept
liability for any errors or omissions in the content of this message, which arise as a result
of e-mail transmission. If verification is required, please request a hard-copy version.
Serono International SA, 15bis Chemin Des Mines, Geneva, Switzerland,

Histonet mailing list 




Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

<< Previous Message | Next Message >>