Re: [Histonet] cleaved caspase-3


I perform caspase-3 routinely using a one hour RT incubation on human xenografts.  I use the Pharmingen primary, and an SABC method.  Let me know if you'd like me to send you the protocol.  However, I am not using formalin fixed tissue - My tissues are zinc fixed paraffin sections.  I haven't been able to get consistent results with caspase-3 on ffpe.

Jacqueline M. O'Connor HT(ASCP)
Abbott Laboratories
Global Pharmaceutical Research and Development
Discovery Chemotheraputics
Fax 847.938.3266

"Linke_Noelle" <>
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09/25/2003 09:37 AM

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        Subject:        [Histonet] cleaved caspase-3

Hi everyone!
Is anyone using cleaved caspase-3 with a protocol other than overnight at 4 degrees?  I'm having problems with my staining and I don't have weeks to get it sorted out, so I am looking for a room temp or heated protocol to try.
Thanks in advance
Noelle Linke, BS, HTL(ASCP)
Allergan, Inc
Irvine, CA 92612

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