I perform caspase-3 routinely using a one hour RT incubation on human xenografts. I use the Pharmingen primary, and an SABC method. Let me know if you'd like me to send you the protocol. However, I am not using formalin fixed tissue - My tissues are zinc fixed paraffin sections. I haven't been able to get consistent results with caspase-3 on ffpe.
Jacqueline M. O'Connor HT(ASCP)
Global Pharmaceutical Research and Development
"Linke_Noelle" <Linke_Noelle@Allergan.com> Sent by: email@example.com
09/25/2003 09:37 AM
Subject: [Histonet] cleaved caspase-3
Is anyone using cleaved caspase-3 with a protocol other than overnight at 4 degrees? I'm having problems with my staining and I don't have weeks to get it sorted out, so I am looking for a room temp or heated protocol to try.
Thanks in advance
Noelle Linke, BS, HTL(ASCP)
Irvine, CA 92612