Re: [Histonet] Re.Isopropanol in processing

From:John Kiernan

Dear All including John D.,

Isopropanol is not miscible with melted
paraffin wax. If you don't believe the 
usual published references you can easily 
check for yourself. Add a drop of melted 
wax to a small beaker containing warm 
isopropyl alcohol. It won't dissolve,
even if you heat to higher than the
melting point of the wax.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1

John Difford wrote:
>  Dear All
> Despite some statements to the contrary that have recently been posted on
> Histonet, I have to inform you that isopropanol is miscible with melted
> paraffin wax and this is why one can use if for processing without the use
> of a clearing agent.
> John Difford
> Histopathology
> Royal Free Hospital
> London
> England, UK

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