Re: [Histonet] Re:Excessively Wrinkled sections

From:"Lee & Peggy Wenk"

Agree with it being OK to raise the temperature of the water. The tissue has
already been through hot paraffin, and, for some labs, will be in a slide
dryer/warmer that is set higher than your current 40 degrees C. flotation

Set the flotation bath about 10 degrees C. BELOW the melting point (mp) of
the paraffin. (Look on side of paraffin container.) Example, if the mp of
the paraffin is 55-58 degrees C., set the flotation bath at 45 - 47 degrees
C. That should take care of the wrinkles.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

----- Original Message -----
From: "Albert Grobe" 
To: ; 
Sent: Friday, September 26, 2003 5:40 PM
Subject: [Histonet] Re:Excessively Wrinkled sections

Since your samples have been through the 60C paraffin, raising the waterbath
temperature a few degrees should not affect you epitopes.  Ideally, the
waterbath should be a couple degrees below the melting temp of the wax but
some fine adjustment is sometimes necessary.  I have had the same problem in
the past, and raising the temp has helped.

Albert C. Grobe, MS
Tissue Engineering Lab
International Heart Institute
Missoula, MT 59802
Office:  (406) 329-5634

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